SpectraMax iD5 Multi-Mode Microplate Reader Optional, for SpectraMax iD5 reader: Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. This unlocks the transport slide. . We would like to show you a description here but the site won’t allow us. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Download PDF. Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Time-. , a. SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. No additional manuals are available. 0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingSpectraMax i3x. com. Access this by going to Help >. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Furthermore, VersaMax system is reliable, which means more uptime and lower long-term cost of ownership. microplate reader. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. SpectraMax M2 and SpectraMax M2 e readers can acquire absorbance as well as fluorescence data for samples by issu ing a single read command in SoftMax. 7°C (gradient heating design). With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax iD5 Multi-Mode Microplate Reader. Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. An enhancer is provided in the kit where applicable to further reduce background fluorescence and to increase the. ATP standard curves read on SpectraMax microplate readers. ” Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. We would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. This technology allows for the automated regulation of well absorbance to a cuvette equivalent path length of 1 cm, which translates to. SpectraMax Spectrofluorometers. 58% for the detection module and 2. degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner. Transcreener ® ADP. The temperature of the microplate chamber can be regulated, if desired from 4°C above ambient to 45°C. Fill a clean plate with water. . Temperature control. The SpectraMax M2 and M2e are versatile and reliable instruments that can perform a range of applications in. Highly customizable low volume. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. Prerequisites: SoftMax Pro 7. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. The SpectraMax L reader is available in 1- and 2-channel (Photomultiplier Tube) configurations. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. The Spectramax 250s xenon flash lamp and grating monochromator facilitates direct UV measurement of DNA, RNA, and protein in microplates. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence intensity. Path Check Pathlength Measurement every Gemini EM Reader. It measures the optical density (OD) of samples in both 96- and 384-well. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. flexibility of monochromator-based systems. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. This kit is optimized for Molecular Devices SpectraMax microplate readers with a preconfigured protocol provided in SoftMax Pro Software for simplified data acquisition and analysis. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. 315 can't find zero order. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). 72 MB. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. Cuvette Microplate wells. The SpectraMax M2 and M2e are versatile and reliable. Examples: “Press carefully to engage,” “ Do not press down. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Multimode Microplate Readers. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. SpectraMax Mini User Guide. It was also read on the monochromator-based. Molecular Devices SpectraDrop ™ Micro-Volume Microplate allows users to read up to 64 samples per plate on SpectraMax ® Microplate Readers, with sample volumes as low as 2 µL. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. Category 2 and 3 microplate readers use gradient heating as well. With optimized reagents, option to operate the. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. 304 signal level saturation. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Read More. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol. 6 for further experiment, so I'm confused which i would choose for OD value determination. Compare plate readersFive-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. For a higher . Add to Cart. Due to elevated security of a Windows 7 64-bit operating system, the USB instrument drivers may not be installed automatically. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. 20 μL of Component A was added to each well of the microplate. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Sale. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeMicroplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. 1 software Download page. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual monochromators. Here, the CVs for each plate were less than 3, and the difference between mean. The fluorometric performance of the SpectraMax M2e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Other technologies such as imaging. With optimized reagents, option to operate the. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. Note that this procedure is only for the SpectraMax L model. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. both cuvette-based and microplate reader applications on the same instrument. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD3 reader. On the back, in the right hand top corner,. 100 fmol 384-well (75 µL) 0. Omnia® Compatible Microplate Reader Documentation Version No. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Or, with the reader specifications noted from the Instrument Description section of your reader's. An assay plate containing a 15-point standard curve was read on the SpectraMax. The dual monochromator optics allow the widest range of. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. The Gemini XPS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The top- and bottom-reading Gemini EM Microplate Reader measures fluorescence on a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well-scan modes. Lift up the lid over the chamber to insert or remove a cuvette. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 0 out of 5 $ 23,995 $ 22,495. The instrument uses a paired monochromator and xenon flash. Or scan up to 384 wells at any time to determine the best assay conditions. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. It can run samples based on pre-defined. Molecular Devices. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. e. SpectraMax® Mini Multi-Mode Microplate Reader for Biotech Laboratories Built on the company’s nearly four decades of experience delivering high-performance microplate reader technology to the market, the SpectraMax Mini reader is robust enough to meet a lab’s core microplate reader needs, while its compact size is ideal for integration into a. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. For the calculation of the ratiometric biosensor signal, the emission intensity at 510 nm was recorded upon an excitation at 380 nm and 470 nm and the former was divided by the latter (Exc. The guide also covers the software features, the specifications, and the accessories of the systems. Detailed specifications of each model reader are reported in Section 7, “Specifications”. Compact designs. We would like to show you a description here but the site won’t allow us. 7°C (gradient heating design). Detection modalities include absorbance (UV-Vis Abs) and. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. See SpectraMax® Microplate Reader User Manual Download Page. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Using the. Read one sample or up to 384 in0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. both cuvette-based and microplate reader applications Microplate Readers. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Wide range of applications. A preconfigured protocol is available in the software to. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The SpectraMax M5e Microplate Reader enables five modes of detection with the additional benefit of being certified for Cisbio Bioassays’ HTRF Assays. Italic and boldface type are used for emphasis. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. The SpectraMax 190 reader can be integrated with Molecular . These robust readers have been placed in labs from. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The CellTiter-Glo assay from Promega uses luciferase. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. . The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. 1) Select the correct plate and the wells that you want to measure. Sensitive fluorescent quantitation of DNA with the Quant-iT. The SpectraMax Mini Multi-Mode Microplate Reader performs your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader. Molecular Interactions. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile. Find regional support. . Additionally, user-installable options allow the SpectraMax i3 System to. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The QuickSync client and automatic USB export option not only frees the user from waiting for data in front of the plate reader, but automatically sends it to one’s computer in an. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Figure 1. com 4. Total volume (except deep. flat meniscus), but is approx 15 mL. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. 5 87. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. no. The plate. : 2 Oct 12 Page 6 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The optical system of the SpectraMax 190 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 850 nm). 5 12. . Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. These instruments often feature additional detection modes in addition to fluorescence intensity, such as UV-vis absorbance, fluorescence. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Choose from a three- (M3), four- (M4), orIn this case, the SpectraMax i3x reader works as a flash-lamp based microplate reader as described in the supporting application note “The AutoPMT™ feature for an extended dynamic range in the SpectraMax iD and M series Multi-Mode Readers”. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Other technologies such as imaging. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Microplate readers’ standard detection modes. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. 300 µL of Detergent Compatible Bradford reagent was added to each well. Step 2: Launch SoftMax Pro Software. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Use similar settings for other Molecular Devices readers with fluorescence detection mode. Reporter Assay. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The 2-channel configuration is available with 0, 2, or 4 injectors. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Path Check Pathlength Measurement SpectraMax i3x. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. keeping with best practices, reader performance should still be validated and documented regularly, especially in regulated laboratories. Spectranet customer portalthe SpectraMax® M Series Multi-Mode Microplate Readers, for . SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. SpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. San Jose, Calif. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Offer Ends In: 18 Days 19: 38: 21. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore ([email protected], high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Unlock your i3/i3x instrument after shipment. Figure 1. edu) to be added as a trained user in iLab. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Assays vary depending on temperature or pH and luminescence microplate reader validation DATA SHEET. 380 nm/Exc. 303 unable to cal dark current. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Italic and boldface type are used for emphasis. 2. Brochure. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. In addition, SoftMax Pro Softwareluminescence, and fluorescence microplate readers, and the SpectraMax® Paradigm® Multi-Mode Detection Platform. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. A SpectraMax M2 microplate reader helps minimize operating time and save costs, allowing researchers to spend more time analyzing data and generating practical knowledge. Materials. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. However, this method is only able to measure down to about 250 ng/mL on a typical absorbance microplate reader. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. It offers the additional advantage of HTRF ® (homogeneous time-resolved fluorescence) certification, allowing. SpectraMax readers are equipped with PathCheck Technology, which measures the pathlength in each well of a microplate and normalizes the absorbance values to a 1-cm pathlength so that they are the same as values obtained in a 1-cm cuvette. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). The chamber contains springs that position the cuvette in the proper alignment for a read. Add to Cart. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Learn more about its features, specifications, and applications in this user. 2 only: SpectraMax Mini Update. We would like to show you a description here but the site won’t allow us. Put 200 µL sample into one or more wells of a microplate and 200 µL buffer or solvent into one or more other wells. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. 5014038F 3 Contents SafetyInformation 5 Warnings,Cautions,Notes,andTips 5 Chapter1:Introduction 11 ComputerIntegration 11 Applications 12 DualPhotomultiplierTubes 12The optical system of SpectraMax Plus384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the UV-visible wavelength range (190 nm - 1000 nm). The 2-channel configuration is available with 0, 2, or 4 injectors. Open the top cover of the instrument. In this product video, see how the SpectraMax M5 quickly reads samples. Ease of use. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. on the SpectraMax iD3 microplate reader Introduction The intrinsic fluorescence of proteins is due to the aromatic amino acids tryptophan, tyrosine, and phenylalanine. DNA and RNA measurements in SpectraMax® microplateSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Cuvette Chamber The cuvette chamber is located to the right of the control panel. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Guides: SpectraMax Mini Installation Guide. . Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. For a higher . their consistent performance, reliability and durability. Table of Contents. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. We would like to show you a description here but the site won’t allow us. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. 306 plate air cal. Blank subtraction and standard curve fitting were done automatically by the software. 2 software: see SoftMax® Pro 7. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. 306 plate air cal fail,low light. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. 020 fmol 0. Next generation SpectraMax® absorbance microplate readers. with SpectraMax Microplate Readers Introduction Double-stranded DNA is typically quantitated in microplate readers by measuring the absorbance of the DNA solution at 260 nm. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 2. SoftMax Pro Software was used to analyze data, and the net ΔRFU values calculated were used to plot a standard curve, generate the R 2 value, and show the sensitivity of this reader in detecting very low endotoxin levels. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. 0 out of 5 $ 5,999. This is equivalent to having 511 built-in filters, The SpectraMax iD5 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the industry. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. Results are shown in Table 1. We would like to show you a description here but the site won’t allow us. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The plate reader can measure multiple readouts, including UV and visible absorbance. Molecular Devices Spectramax iD3 Microplate Reader. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1 nm increments. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. zip. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The temperature in the plate chamber is ambient and isothermal. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 33 Dramatic aggregation of Alzheimer A b by Cu(II) is induced by conditions representing physiological acidosis . Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. begin reading microplates with seven mouse clicks. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Step 3: Use the Template Editor in the cell standards section of the protocol to create a template of the assay indicating the location of standards (cell numbers for each dilution. The SpectraMax® ABS Plus Microplate Reader can run . SAN JOSE, CA — Molecular Devices, LLC. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Use the provided 3. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerThe Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax® ABS Plus readers use a grating monochromator to select the exact wavelength needed for every assay. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. The following document is intended to demonstrate setup of this instrument. 0 out of 5 $ 2,999-20%. Fluorescence Microplate Reader Comparison Chart. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Figure 6: Transcreener ADP2 FI assay principle. The plate reader optics are tunable for absorbance. Figure 3. SpectraMax Microplate Readers. Example: [Drawer]. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Microplate automation and detection instruments for walk away operation and high quality results. 0 out of 5The SpectraMax ® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. Use the provided 3. Assays can be better optimized by scanning across. 7°C and the bottom to 36. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The plate reader can measure multiple readouts, including UV. Our specialization servicing. Microplate spectrophotometers, also called microplate readers, allow researchers to analyze multiple samples in parallel using 6- to 1536-well formats, as opposed to single sample measurements. 20 μL of Component A was added to each well of the microplate. The dual monochromator optics allow.